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Isolierung von CD146
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Isolierung von CD146

Article DOI: 10.3791/53782-v • 09:47 min • June 17th, 2016
June 17th, 2016 •
1,2, 1,3,4, 1,2,5
1Sinclair Centre for Regenerative Medicine, Ottawa Hospital Research Institute, 2University of Ottawa, 3Department of Neonatology and Pediatric Critical Care Medicine, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, 4DFG Research Center and Cluster of Excellence for Regenerative Therapies (CRTD), Technische Universität, Dresden, 5Children's Hospital of Eastern Ontario Research Institute

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Dieses Protokoll beschreibt ein Isolationstechnik für primäre Lungen resident mesenchymalen Stromazellen von Ratten erhalten, durch die Verwendung von enzymatischen Verdauung, Dichtegradiententrennung, Kunststoff Haftung und CD146 + magnetisches Kügelchen Auswahl.

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Stem Cell Biology Ausgabe 108 mesenchymale Stromazellen Gewebe ansässig Stamm- / Vorläuferzellen Lunge Ratte regenerative Medizin Magnetic-Bead-Auswahl
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