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JoVE Journal
Neuroscience

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设计,包装,并通过立体定向注射高效价CRISPR复古和慢交付
 
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设计,包装,并通过立体定向注射高效价CRISPR复古和慢交付

Article DOI: 10.3791/53783-v 11:28 min May 23rd, 2016
May 23rd, 2016

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

该CRISPR / Cas9系统提供作出有针对性的基因组编辑获得和负担得起科学界的潜力。该协议旨在演示如何创建病毒将基因敲除使用CRISPR / Cas9系统感兴趣的基因,然后立体定位将它们注入成年小鼠大脑。

Tags

神经科学,第111,慢病毒,逆转录病毒,病毒包装,滴度高,
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