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JoVE Journal Developmental Biology
Feeder-vrij Afleiding van melanocyten uit menselijke pluripotente stamcellen
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Feeder-vrij Afleiding van melanocyten uit menselijke pluripotente stamcellen

Article DOI: 10.3791/53806 • 12:21 min • March 3rd, 2016
March 3rd, 2016 •
1,2, 3, 1
1The Center for Stem Cell Biology, Developmental Biology Program, Memorial Sloan-Kettering Cancer Center, 2Cancer Biology and Genetics Program, Gerstner Sloan-Kettering Graduate School, Sloan-Kettering Institute for Cancer Research, 3Thermo Fisher Scientific

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Dit werk beschrijft een in vitro differentiatie protocol bij gepigmenteerde, volwassen melanocyten uit menselijke pluripotente stamcellen te produceren via een neurale en melanoblast tussenstap met behulp van een feeder-vrij, 25 dagen protocol.

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Developmental Biology melanocyten pigmentatie melanoblast embryonale stamcellen (SER) de menselijke pluripotente stamcellen (hPSCs) geïnduceerde pluripotente stamcellen (iPSCs) Neurale Crest in vitro differentiatie ziekte modelleren differentiatie protocol humane embryonale stamcellen
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