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Neuroscience

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JoVE Journal Neuroscience
用优化的n-甲基 d-葡乙胺保护恢复法制备急性脑切片
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用优化的n-甲基 d-葡乙胺保护恢复法制备急性脑切片

Article DOI: 10.3791/53825-v • 10:53 min • February 26th, 2018
February 26th, 2018 •
*1, *1, 1, 1, 2, 1, 1, 1
1Cell Types Program, Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute
* These authors contributed equally

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该协议演示了一种优化的N-甲基 D-葡乙胺 (NMDG) 脑切片制备保护恢复方法的实现。一个单一的媒体配方是用来可靠地获得健康的脑切片从动物的任何年龄和不同的实验应用。

Tags

神经科学 问题 132 脑切片 补丁钳 电生理学 NMDG 保护性恢复方法 突触连接 大脑皮层 Na 钉入 多神经元记录
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