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High-throughput CRISPR Vector Konstruktion og karakterisering af DNA Ændringer af Generation of Tomato Behårede Roots
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High-throughput CRISPR Vector Konstruktion og karakterisering af DNA Ændringer af Generation of Tomato Behårede Roots

Article DOI: 10.3791/53843-v • 12:59 min • April 30th, 2016
April 30th, 2016 •
1, 1,2
1Boyce Thompson Institute for Plant Research, 2Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science, Cornell University

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Anvendelse af DNA samling, kan flere CRISPR vektorer konstrueres parallelt i en enkelt kloning reaktion, hvilket gør konstruktionen af ​​et stort antal CRISPR vektorer en enkel opgave. Tomat hårrødder er en glimrende modelsystem til at validere CRISPR vektorer og generere mutant materialer.

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Molekylærbiologi , CRISPR / Cas9 genteknologi genom redigering DNA forsamling plantebioteknologi transformation plante
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