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Haut-débit CRISPR Vector Construction et caractérisation de l'ADN Modifications par génération de tomate Poilu Roots
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Haut-débit CRISPR Vector Construction et caractérisation de l'ADN Modifications par génération de tomate Poilu Roots

Article DOI: 10.3791/53843 • 12:59 min
April 30th, 2016 •
1, 1,2
1Boyce Thompson Institute for Plant Research, 2Section of Plant Pathology & Plant-Microbe Biology, School of Integrative Plant Science, Cornell University

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April 30th, 2016

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Utilisation de l'assemblage de l'ADN, de multiples vecteurs de CRISPR peuvent être construits en parallèle dans une réaction de clonage unique, ce qui rend la construction d'un grand nombre de vecteurs CRISPR une tâche simple. Tomate racines chevelues sont un excellent modèle pour valider des vecteurs CRISPR et de générer des matériaux mutantes.

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