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Produksjon av menneske norovirus utstående domener i
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Produksjon av menneske norovirus utstående domener i

Article DOI: 10.3791/53845-v • 10:46 min • April 19th, 2016
April 19th, 2016 •
1,2, 1,2, 1,2, 1,2
1Schaller Research Group, University of Heidelberg and the German Cancer Research Center, 2Department of Infectious Diseases, Virology, University of Heidelberg

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Her beskriver vi en metode for å uttrykke og rense høy kvalitet norovirus utstå (P) domener i E. coli for anvendelse ved røntgenkrystallografi studier. Denne fremgangsmåten kan anvendes for andre calicivirus P domener, så vel som ikke-strukturelle proteiner, f.eks., Viralt genom proteinbundet (VPG), protease, og RNA-avhengig RNA-polymerase (RdRp).

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Molecular Biology Cloning norovirus P domene protein uttrykk protein rensing krystallisering
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