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En simpel Bioassay for Vurdering af Vascular endotelvækstfaktorer

Article DOI: 10.3791/53867-v • 09:04 min • March 15th, 2016
March 15th, 2016 •
1, 1, 1, 1, 1
1Tumour Angiogenesis Program, Peter MacCallum Cancer Centre

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Vi beskriver en simpel cellebaseret bioassay til påvisning, kvantificering og overvågning af aktiviteten af ​​medlemmer af den vaskulære endotelvækstfaktor familien af ​​ligander. Assayet anvender kimære receptorer udtrykt i en faktor-afhængig cellelinie at tilvejebringe en semi-kvantitativ eller kvantitativ vurdering af receptorbinding og tværbinding af liganden.

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Cellular Biology Receptor VEGF ligand inhibitor signalering opløselige receptorer protein-vækstfaktor bioassay monoklonalt antistof
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