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LC-MS analyse av humane blodplater som en plattform for å studere mitokondrie Metabolism
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LC-MS analyse av humane blodplater som en plattform for å studere mitokondrie Metabolism

Article DOI: 10.3791/53941-v • 06:04 min • April 4th, 2016
April 4th, 2016 •
*1,2, *2,3, 1,2, 2,3, 2,3, 4, 4, 1,2, 5, 1,2
1Center for Cancer Pharmacology, University of Pennsylvania, 2Center for Excellence in Environmental Toxicology, University of Pennsylvania, 3Penn SRP and Department of Systems Pharmacology and Translational Therapeutics, University of Pennsylvania, 4Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery, University of Pennsylvania, 5A.J. Drexel Autism Institute, Drexel University
* These authors contributed equally

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Her viser vi isolerte humane blodplater kan anvendes som en rullestol ex vivo modell for å studere metabolske tilpasninger som reaksjon på det komplekse I-inhibitor rotenon. Denne tilnærmingen benytter isotopisk sporing og relativ kvantifisering ved væske-kromatografi-massespektrometri og kan brukes på en rekke forskjellige studiedesign.

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Environmental Sciences Metabolism isotoper sporstoffer blodplater massespektrometri mitokondrier rotenon xenobiotics
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