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JoVE Journal Chemistry
Immobilisering af Multi-biokatalysatorer i alginatkugler for cofaktor Regeneration og Forbedret Genbrugelighed
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Immobilisering af Multi-biokatalysatorer i alginatkugler for cofaktor Regeneration og Forbedret Genbrugelighed

Article DOI: 10.3791/53944-v • 09:27 min • April 22nd, 2016
April 22nd, 2016 •
1, 2, 1, 2
1Department of Chemical Engineering, Konkuk University, 2Department of Chemical Engineering, University of Michigan

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Præsenteret er protokollen for co-immobilisering hel-celle biokatalysatorer til cofaktor regenerering og forbedret genanvendelighed, ved hjælp af produktion af L-xylulose som eksempel. Cofaktoren regenerering opnås ved kobling to Escherichia coli stammer, der udtrykker funktionelt komplementære enzymer; helcelle-biokatalysator immobilisering opnås ved celleindkapsling i calciumalginatperler.

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Kemi helcelle biokatalysator cellefri ekstrakt cofaktor regenerering enzym-koblet system immobilisering L-xylulose multi-protein synergi
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