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JoVE Journal
Chemistry

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藻酸盐珠多生物催化剂辅因子再生和可重用性改进的固定化
 
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藻酸盐珠多生物催化剂辅因子再生和可重用性改进的固定化

Article DOI: 10.3791/53944-v 09:27 min April 22nd, 2016
April 22nd, 2016

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

呈现为共固定化全细胞生物催化剂用于辅因子再生和改善的可重用性,使用生产L-木酮糖的作为例子的协议。辅因子再生是通过偶合表达功能上互补酶2 大肠杆菌菌株实现;全细胞生物催化剂固定化是通过细胞封装在藻酸钙珠来实现。

Tags

化学,第110,全细胞生物催化剂,无细胞提取物,辅因子再生,酶偶联系统,固定化,L-木酮糖,多蛋白的协同作用
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