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Journal / Developmental Biology / Methoden zur Untersuchung MRP4- haltigen…
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Developmental Biology

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JoVE Journal Developmental Biology
Methoden zur Untersuchung MRP4- haltigen Makromolekulare Komplexe in der Verordnung von Fibroblast Migration
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Methoden zur Untersuchung MRP4- haltigen Makromolekulare Komplexe in der Verordnung von Fibroblast Migration

Article DOI: 10.3791/53973-v • 10:43 min • May 19th, 2016
May 19th, 2016 •
*1,2, *1, 1,2
1Division of Pulmonary Medicine, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center, 2Department of Physiology, University of Tennessee Health Science Center
* These authors contributed equally

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MRP4- regelt verschiedene zyklische Nukleotid-abhängige Signalereignisse einschließlich einer kürzlich aufgeklärte Rolle bei der Zellmigration. Wir beschreiben eine direkte, aber vielschichtigen Ansatz, um die nachgelagerten molekularen Targets von MRP4- zu entwirren bei der Identifizierung eines einzigartigen MRP4- Interaktom führt, die Schlüsselrollen in der fein abgestimmten Regulierung der Fibroblasten-Migration spielt.

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Entwicklungsbiologie Heft 111 Multidrug-Resistenzprotein 4 (MRP4-) Actin cAMP-abhängige Proteinkinase (PKA) Migration Ingenuity Pathway Analysis (IPA) High-Content-Mikroskopie Förster-Resonanzenergietransfer (FRET).
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