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Preparazione del 3D collagene Gel e microcanali per lo studio delle interazioni 3D

Article DOI: 10.3791/53989-v • 10:24 min • May 9th, 2016
May 9th, 2016 •
1,2, 1, 1, 1, 2,3,4, 1,2,5
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison, 2Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, 3Department of Biomedical Engineering, University of Wisconsin-Madison, 4Morgridge Institute for Research, University of Wisconsin-Madison, 5Paul P. Carbone Comprehensive Cancer center, University of Wisconsin-Madison

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Il collagene è una componente fondamentale della ECM, e fornisce spunti essenziali per molti processi cellulari che vanno dalla migrazione verso la differenziazione e la proliferazione. Qui fornito è un protocollo per l'incorporamento celle all'interno di idrogel di collagene 3D, e una tecnica più avanzata per la generazione di matrici di collagene randomizzati o allineati con microcanali PDMS.

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Bioingegneria collagene canali microfluidica matrici 3D ECM l'allineamento del collagene microambiente tumorale
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