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Journal / Genetics / siRNA Transfektion och EMSA Analyser av nyligen…
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Genetics

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JoVE Journal Genetics
siRNA Transfektion och EMSA Analyser av nyligen isolerade mänskliga villös cytotrofoblaster
Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
Journal (Genetics)
Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
dATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA
Journal (Genetics)
dATP-Tailing, Adapter Ligation, and PCR Amplification of Scarce Cell DNA
 
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siRNA Transfektion och EMSA Analyser av nyligen isolerade mänskliga villös cytotrofoblaster

Article DOI: 10.3791/53995-v • 09:57 min • September 20th, 2016
September 20th, 2016 •
1, 2, 3, 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

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Detta protokoll beskriver en metod för att effektivt transfektera siRNA i färsk isolerade humana villösa cytotrofoblaster genom att använda mikroporation och identifiera DNA-protein-komplex i dessa celler. Transfekterade celler kan övervakas med Western blot och EMSA-analyser under 4-dagars odling tid.

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Genetik molekylärbiologi mänskliga villösa cytotrofoblaster Syncytin-2 siRNA mikroporation Lipid-baserade transfektion Elektro Mobility Shift Assay
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