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JoVE Journal Genetics
siRNA Transfection et EMSA Analyses sur fraîchement isolés villosités cytotrophoblastes humaines
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siRNA Transfection et EMSA Analyses sur fraîchement isolés villosités cytotrophoblastes humaines

Article DOI: 10.3791/53995-v • 09:57 min • September 20th, 2016
September 20th, 2016 •
1, 2, 3, 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

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Ce protocole décrit un procédé pour transfecter efficacement ARNsi dans les cytotrophoblastes villeux humaines fraîchement isolées en utilisant la microporation et l'identification des complexes ADN-protéine dans ces cellules. Les cellules transfectées peuvent être contrôlées par Western Blot et des analyses EMSA pendant la période de culture de 4 jours.

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Genetics numéro 115 biologie moléculaire numéro cytotrophoblastes villeux humaines syncytine-2 siRNA Microporation transfection à base de lipides de mobilité électrophorétique Maj Assay
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