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Expression and Purification of Virus-like Particles for Vaccination
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Expression and Purification of Virus-like Particles for Vaccination

Article DOI: 10.3791/54041-v • 06:17 min • June 2nd, 2016
June 2nd, 2016 •
1, 1, 1
1Center for Vaccines and Immunology, Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia

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Summary
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Here, we present a protocol for synthesizing virus-like particles using either baculovirus or mammalian expression systems, and ultracentrifugation purification. This highly customizable approach is used to identify viral antigens as vaccine targets in a safe and flexible manner.

Tags

Virus-like Particles VLPs Expression Purification Mammalian Cell Expression System Insect-cell Expression System Viral Antigens Protein Precipitation Polyethylene Glycol Density Layers Vaccine Targets Disease Diagnosis Serology Glycerol Underlay Transvection Liposome DNA Solutions
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