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Journal / Immunology and Infection / Ett-trinns Negativ kromatografiske rensing i
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Immunology and Infection

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JoVE Journal Immunology and Infection
Ett-trinns Negativ kromatografiske rensing i
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Ett-trinns Negativ kromatografiske rensing i

Article DOI: 10.3791/54043-v • 10:44 min • June 18th, 2016
June 18th, 2016 •
1, 1, 1, 1, 1,2
1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University

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Et høyt utbytte Fremgangsmåte for ett-trinns negativ rensing av rekombinant Helicobacter pylori nøytrofil-aktiverende protein (HP-NAP) overuttrykt i Escherichia coli ved hjelp av dietylaminoetyl harpikser i batch-modus, er beskrevet. HP-NAP renset ved denne fremgangsmåte er fordelaktig for utvikling av vaksiner, medikamenter, eller diagnostikk for H. pylori -associated sykdommer.

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Immunologi HP-NAP, DEAE Negativ kromatografi ubundne fraksjonen Batch kromatografi,
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