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JoVE Journal Developmental Biology
Mikroinjektion for Transgenese og Genome Redigering i Trepigget Sticklebacks
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Mikroinjektion for Transgenese og Genome Redigering i Trepigget Sticklebacks

Article DOI: 10.3791/54055-v • 08:51 min • May 13th, 2016
May 13th, 2016 •
1, 1, 1
1Department of Molecular and Cell Biology, University of California, Berkeley

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Transgene manipulationer og genom-redigering er kritiske for funktionelt teste roller gener og cis reguleringsmyndigheder elementer. Her et detaljeret mikroinjektion protokol for generering af genomiske modifikationer (herunder Tol2-medierede fluorescerende reporter transgene konstruktioner, Talens og CRISPRs) præsenteres for emergente model fisk, den trepigget hundestejle.

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Developmental Biology hundestejle mikroinjektion Talen CRISPR genom redigering Tol2 forstærker,
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