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JoVE Journal
Developmental Biology

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显微注射转基因的基因组,并在编辑Threespine刺鱼
 
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显微注射转基因的基因组,并在编辑Threespine刺鱼

Article DOI: 10.3791/54055-v 08:51 min May 13th, 2016
May 13th, 2016

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Summary

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转基因操作和基因组编辑是功能测试基因和 -regulatory元素的作用是至关重要的。此处的基因组修饰(包括TOL2介导的荧光报道基因构建体,TALENS和CRISPRs)的生成的详细显微注射协议提出了紧急模型鱼的三刺。

Tags

发育生物学,第111,刺,显微注射,TALEN,CRISPR,基因组编辑,TOL2,增强剂,
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