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Developmental Biology

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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
 
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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Article doi: 10.3791/54055
May 13th, 2016

Summary May 13th, 2016

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Transgenic manipulations and genome editing are critical for functionally testing the roles of genes and cis-regulatory elements. Here a detailed microinjection protocol for the generation of genomic modifications (including Tol2-mediated fluorescent reporter transgene constructs, TALENs, and CRISPRs) is presented for the emergent model fish, the threespine stickleback.

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