El estudio de la Organización Supramolecular de membranas fotosintéticas dentro de los tejidos foliares fracturados-Freeze por Cryo-Microscopía Electrónica de Barrido

Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.