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Modelando Myotonic distrofia 1 em células C2C12 de mioblastos
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Modelando Myotonic distrofia 1 em células C2C12 de mioblastos

Article DOI: 10.3791/54078-v • 09:39 min • July 29th, 2016
July 29th, 2016 •
*1, *1,3, 1, 1,3, 1,2, 1
1Department of Biology and Biochemistry, University of Houston, 2Department of Economics, University of Houston, 3Department of Cardiology, The First Affiliated Hospital of Nanchang University
* These authors contributed equally

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Neste protocolo, apresentamos os procedimentos para o estabelecimento de modelos de mioblastos distrofia miotônica 1, incluindo otimizado manutenção C2C12 celular, transfecção gene / transdução e diferenciação dos miócitos.

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Medicine 113 Edição distrofia miotónica C2C12 de mioblastos Celf1 transfecção de plasmídeo a transdução retroviral transdução lentiviral imunocoloração
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