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Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Article DOI: 10.3791/54093-v • 11:35 min • August 21st, 2016
August 21st, 2016 •
1, 1,2,3, 1,3, 1, *1,4, *1
1Center for Autoimmune Genomics and Etiology, Cincinnati Children's Hospital, 2Medical Scientist Training Program, University of Cincinnati, 3Immunology Graduate Program, University of Cincinnati, 4Divisions of Biomedical Informatics and Developmental Biology, Cincinnati Children's Hospital
* These authors contributed equally

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Summary
Automatic Translation

We present a strategic plan and protocol for identifying non-coding genetic variants affecting transcription factor (TF) DNA binding. A detailed experimental protocol is provided for electrophoretic mobility shift assay (EMSA) and DNA affinity precipitation assay (DAPA) analysis of genotype-dependent TF DNA binding.

Tags

Functional Non-coding Genetic Variants Electrophoretic Mobility Shift Assay EMSA DNA-affinity Precipitation Assay DAPA Genomics Disease Phenotype Genetic Variance Regulatory Proteins Pathways Candidate Causal Variant Nuclear Lysates B-lymphoblastoid Cells Cell Lines
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