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Isolamento di gigante Lampbrush cromosomi da Living ovociti di rane e salamandre
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Isolamento di gigante Lampbrush cromosomi da Living ovociti di rane e salamandre

Article DOI: 10.3791/54103-v • 10:07 min • December 5th, 2016
December 5th, 2016 •
1, 1
1Department of Embryology, Carnegie Institution for Science

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Vi presentiamo semplici tecniche per isolare giganti cromosoma a spazzola trascrizionalmente attivi da ovociti di rane e salamandre vivono. Descriviamo come osservare questi cromosomi "vivo" di contrasto di fase o differenziale contrasto interferenziale, e come risolverli per fluorescente ibridazione in situ o di immunofluorescenza colorazione.

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Genetica Germinal vescicole (GV) istone corpo locus cromosoma a spazzola involucro nucleare macchiolina nucleare nucleolo ovocita trascrizione
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