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Journal / Genetics / Isolement du géant lampbrush Chromosomes de…
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JoVE Journal Genetics
Isolement du géant lampbrush Chromosomes de Living oocytes de grenouilles et salamandres
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Isolement du géant lampbrush Chromosomes de Living oocytes de grenouilles et salamandres

Article DOI: 10.3791/54103-v • 10:07 min • December 5th, 2016
December 5th, 2016 •
1, 1
1Department of Embryology, Carnegie Institution for Science

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Nous présentons des techniques simples pour isoler géants chromosomes plumeux transcriptionnellement actifs à partir d'ovocytes de grenouilles et de salamandres vivant. Nous décrivons comment observer ces chromosomes "vivant" par contraste de phase ou de contraste d' interférence différentiel, et comment les fixer pour hybridation in situ fluorescente ou immunofluorescence dans.

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Genetics numéro 118 germinal vésiculaire (GV) le corps de locus histone chromosome lampbrush enveloppe nucléaire chatoiement nucléaire nucléole ovocyte transcription
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