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JoVE Journal
Genetics

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从青蛙和蝾螈的卵母细胞活巨Lampbrush染色体的分离
 
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从青蛙和蝾螈的卵母细胞活巨Lampbrush染色体的分离

Article DOI: 10.3791/54103-v 10:07 min December 5th, 2016
December 5th, 2016

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Summary

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Click here for the English version.

我们提出简单的技术从活青蛙和蝾螈卵母细胞中分离巨头转录活性lampbrush染色体。我们描述了如何通过相位对比或微分干涉对比,以及如何解决这些问题原位杂交和免疫荧光染色荧光观察这些染色体“活着”。

Tags

遗传学,118期,生发泡(GV),组蛋白基因的身体,lampbrush染色体,核膜,核去斑,核仁,卵母细胞,转录
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