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Journal / Genetics / Isolering av Giant Lampbrush Kromosomer fra…
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JoVE Journal Genetics
Isolering av Giant Lampbrush Kromosomer fra Living oocytter av frosker og salamandere
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Isolering av Giant Lampbrush Kromosomer fra Living oocytter av frosker og salamandere

Article DOI: 10.3791/54103-v • 10:07 min • December 5th, 2016
December 5th, 2016 •
1, 1
1Department of Embryology, Carnegie Institution for Science

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Vi presenterer enkle teknikker for å isolere gigantiske transcriptionally aktive lampbrush kromosomer fra å leve oocytter av frosker og salamandere. Vi beskriver hvordan observere disse kromosomene "live" av fase kontrast eller differensial interferens kontrast, og hvordan du løser dem for fluorescerende in situ hybridisering eller immunofluorescent farging.

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Genetikk Germinal vesikkel (GV) histon locus kroppen lampbrush kromosom kjernekraft konvolutt kjernekraft flekk kjerne oocytten transkripsjon
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