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Bioengineering

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JoVE Journal Bioengineering
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
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A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Article DOI: 10.3791/54105-v • 09:51 min • June 16th, 2016
June 16th, 2016 •
1,2, 1,2, 1,2,3
1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Taiwan, 2Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3Institute of NanoEngineering and MicroSystems, National Tsing Hua University

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Summary
Automatic Translation

Here, we present a protocol for isolating and culturing single cells with a microfluidic platform, which utilizes a new microwell design concept to allow for high-efficiency single cell isolation and long-term clonal culture.

Tags

Microfluidic Platform High-throughput Single-cell Isolation Culture Fabrication Operation Microfluidic Chip Efficiency Useful Tool Single-cell Isolation And Culture Technique Loading Single Cells Microwell Arrays Gentle Flow Gravitational Force Minimizing Cell Damage Implication Human Disease Diagnosis Cancer Research Cellular Heterogeneity Developing Method Microwell Cell Line Establishment Time-consuming Inefficient Individual Cell Analysis Observation Of Cell Course And Behavior Fabricate Silanized Master Molds Photolithography Method
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