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Journal / Biology / FRET Imaging en hidrogeles tridimensionales
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Biology

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JoVE Journal Biology
FRET Imaging en hidrogeles tridimensionales
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FRET Imaging en hidrogeles tridimensionales

Article DOI: 10.3791/54135-v • 09:47 min • August 1st, 2016
August 1st, 2016 •
*1, *1,3, 1,2
1Department of Oral Biology, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, 2Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, 3Laerdal AS
* These authors contributed equally

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formación de imágenes por resonancia Förster transferencia de energía (FRET) es una poderosa herramienta para estudios de biología celular en tiempo real. Aquí se presenta un método para las células de formación de imágenes de FRET en fisiológicos microambientes tridimensional (3D) de hidrogel utilizando microscopía de epifluorescencia convencional. Se describe un análisis de sondas FRET ratiometric que produce coeficientes lineal en el intervalo de activación.

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Biología Molecular No. 114 microfluidos microtejidos fluorescencia Förster andamio polietilenglicol hidrogel FRET la señalización intracelular los condrocitos
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