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JoVE Journal
Biology

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FRET Imaging i Tre-dimensionelle Hydrogeler
 

FRET Imaging i Tre-dimensionelle Hydrogeler

Article doi: 10.3791/54135
August 1st, 2016 Usage Statistics

Summary August 1st, 2016

Please note that all translations are automatically generated.

Click here for the English version.

Förster resonans (FRET) imaging er et kraftfuldt værktøj til realtid cellebiologiske undersøgelser. Her en fremgangsmåde til FRET billeddannelse celler i fysiologiske tredimensionale (3D) hydrogel mikromiljøer anvendelse af traditionel epifluorescensmikroskopi præsenteres. En analyse for ratiometriske FRET sonder, der giver lineære nøgletal over aktiveringen sortiment er beskrevet.

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