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Journal / Biology / FRET Imaging i Tredimensjonal Hydrogeler
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Biology

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FRET Imaging i Tredimensjonal Hydrogeler
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FRET Imaging i Tredimensjonal Hydrogeler

Article DOI: 10.3791/54135-v • 09:47 min • August 1st, 2016
August 1st, 2016 •
*1, *1,3, 1,2
1Department of Oral Biology, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, 2Department of Bioengineering, Center for Craniofacial Regeneration, McGowan Institute of Regenerative Medicine, University of Pittsburgh, 3Laerdal AS
* These authors contributed equally

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Förster resonans energi overføring (FRET) bildebehandling er et kraftig verktøy for sanntidscellebiologi studier. Her en fremgangsmåte for å FRET avbildnings cellene i fysiologiske tre-dimensjonale (3D) hydrogel microenvironments ved hjelp av konvensjonelle epifluorescens mikroskopi er presentert. En analyse for Proporsjonellekspansjon FRET prober som gir lineære forholdstall enn aktiveringsområdet er beskrevet.

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Molecular Biology mikrofluid microtissue fluorescens Förster stillas polyetylenglykol hydrogel gnage intracellulær signalering chondrocytes
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