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Journal / Developmental Biology / Isolering af murint embryonale Hemogenic…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolering af murint embryonale Hemogenic endotelceller
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Isolering af murint embryonale Hemogenic endotelceller

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016
June 17th, 2016 •
*1, *2, 3, 1
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Hæmatopoietiske stamceller og stamceller (HSPC) stammer fra specialiserede (hemogenic) endotelceller under udvikling, men alligevel lidt om den proces, hvorved nogle endotelceller angiver at blive bloddannende. Vi demonstrerer et flow-cytometri metode tillader samtidig isolering af hemogenic endothelceller og HSPC fra murine embryonale væv.

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Developmental Biology hemogenic endotel endelig bloddannelsen vaskulær udvikling musefostre FACS bloddannende stamceller og stamceller
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