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Journal / Developmental Biology / Isolamento di cellule murine embrionali Hemogenic …
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Developmental Biology

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JoVE Journal Developmental Biology
Isolamento di cellule murine embrionali Hemogenic endoteliali
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Isolamento di cellule murine embrionali Hemogenic endoteliali

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016
June 17th, 2016 •
*1, *2, 3, 1
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Le cellule staminali e progenitrici ematopoietiche (HSPC) derivano da (hemogenic) cellule endoteliali specializzate durante lo sviluppo, ma poco si sa circa il processo attraverso il quale alcune cellule endoteliali specificano a diventare la formazione del sangue. Dimostriamo un metodo basato citometria a flusso che permette l'isolamento simultaneo di cellule endoteliali hemogenic e HSPC da tessuti embrionali murine.

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Biologia dello Sviluppo Numero 112 endotelio hemogenic ematopoiesi definitivo sviluppo vascolare embrioni di topo FACS le cellule staminali e progenitrici ematopoietiche
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