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Immunology and Infection

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JoVE Journal Immunology and Infection
Assay van Adhesie Under Shear Stress voor de Studie van T-lymfocyten-Adhesie Molecule Interacties
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Assay van Adhesie Under Shear Stress voor de Studie van T-lymfocyten-Adhesie Molecule Interacties

Article DOI: 10.3791/54203 • 07:40 min • June 29th, 2016
June 29th, 2016 •
1, 1, 1, 1,2
1Department of Medicine, New York University School of Medicine, 2Department of Pathology, New York University School of Medicine

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Deze stroom adhesietest een eenvoudige, high impact model van T-cel-epitheliale cel interacties. Een spuitpomp gebruikt om afschuifspanning te genereren en confocale microscopie neemt beelden voor kwantificering. Het doel van deze studies is om effectief te kwantificeren T-cel adhesie met behulp van stroom omstandigheden.

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Immunologie T-lymfocyten hechting stroom schuifspanning LFA-1 ICAM-1 Rap1.
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