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Nachweis von RNA-bindenden Proteinen durch
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Nachweis von RNA-bindenden Proteinen durch

Article DOI: 10.3791/54207-v • 10:34 min • July 22nd, 2016
July 22nd, 2016 •
*1, *1, 3, 1,2
1Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 2Institute of Molecular and Cell Biology, Singapore, 3Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University
* These authors contributed equally

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Ein RNA-Pull-down-Protokoll wird für den Nachweis von Interaktionen zwischen RNA-Bindeproteine ​​(RBPs) und nicht-codierende sowie kodierenden RNAs hier optimiert. Ein RNA-Fragment von Androgen-Rezeptor (AR) wurde als Beispiel verwendet, zu zeigen, wie ihre RBP aus lystate primärer braunen Fettzellen zu erhalten.

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Molecular Biology Ausgabe 113 RNA Pull-down RNA-bindendes Protein Adipozyten nichtkodierende Codierung
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