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Journal / Biology / Påvisning av RNA-bindende proteiner etter
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Påvisning av RNA-bindende proteiner etter
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Påvisning av RNA-bindende proteiner etter

Article DOI: 10.3791/54207-v • 10:34 min • July 22nd, 2016
July 22nd, 2016 •
*1, *1, 3, 1,2
1Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 2Institute of Molecular and Cell Biology, Singapore, 3Division of Bioengineering, School of Chemical & Biomedical Engineering, Nanyang Technological University
* These authors contributed equally

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En RNA pull-down-protokollen er optimalisert her for påvisning av vekselvirkninger mellom RNA-bindende proteiner (RBPs) og ikke-kodende samt koding RNA. Et RNA-fragment fra androgenreseptoren (AR) ble anvendt som et eksempel for å vise hvordan man skal hente sine RBP fra lystate av primære brune adipocytter.

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Molecular Biology RNA pull-down RNA-bindende protein fettceller noncoding koding
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