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Chapters
- 00:05Title
- 00:54Brain Tissue Preparation
- 03:32SBFSEM Imaging
- 04:15Analyzing the Imaging Dataset
- 05:43Results: Analysis of Brain Mitochondrial Morphology, Distribution, Abundance and Ultrastructure Using Serial Block-Face Scanning Electron Microscopy
- 07:04Conclusion
Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.
