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JoVE Journal Developmental Biology
Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
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Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Article DOI: 10.3791/54219-v • 09:34 min • July 30th, 2016
July 30th, 2016 •
1, 1, 1, 2,4, 2,5, 2,3, 1,2
1Department of Plant and Wildlife Sciences, Brigham Young University, 2Department of Entomology, Cornell University, 3Department of Molecular Biology and Genetics, Cornell University, 4Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, 5Biological Sciences, SUNY Oswego

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Summary
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A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.

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Fruit Fly Drosophila Melanogaster Axenic Conditions Gnotobiotic Conditions Microbiota Sterilization Rinsing Agar Plates Anaerobic Cultures Carbon Dioxide Lactobacillus Species Acetobacter Species Sterile Diet Erlenmeyer Flask Microwave Boil Stirring Conical Centrifuge Tubes Autoclavable Polypropylene Rack
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