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Developmental Biology

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Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions
 

Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions

Article doi: 10.3791/54219
July 30th, 2016

Summary July 30th, 2016

A method for rearing Drosophila melanogaster under axenic and gnotobiotic conditions is presented. Fly embryos are dechorionated in sodium hypochlorite, transferred aseptically to sterile diet, and reared in closed containers. Inoculating diet and embryos with bacteria leads to gnotobiotic associations, and bacterial presence is confirmed by plating whole-body Drosophila homogenates.

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