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Journal / Developmental Biology / Aufzucht der Fruit Fly
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JoVE Journal Developmental Biology
Aufzucht der Fruit Fly
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Aufzucht der Fruit Fly

Article DOI: 10.3791/54219-v • 09:34 min • July 30th, 2016
July 30th, 2016 •
1, 1, 1, 2,4, 2,5, 2,3, 1,2
1Department of Plant and Wildlife Sciences, Brigham Young University, 2Department of Entomology, Cornell University, 3Department of Molecular Biology and Genetics, Cornell University, 4Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, 5Biological Sciences, SUNY Oswego

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Verfahren zur Aufzucht von Drosophila melanogaster unter axenic und gnotobiotischer Bedingungen vorgestellt. Fly Embryonen werden in Natriumhypochlorit, aseptisch in sterile Ernährung dechorionated und aufgezogen in geschlossenen Behältern. Impfen Ernährung und Embryonen mit Bakterien führt zu gnotobiotischer Verbänden und bakterielle Präsenz wird durch Plattierung Ganzkörper- Drosophila Homogenate bestätigt.

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Entwicklungsbiologie Heft 113 axenic gnotobiotischer, Microbiome Mikrobiota,
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