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JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Opdræt bananfluen
 
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Opdræt bananfluen

Article doi: 10.3791/54219
July 30th, 2016 •
1, 1, 1, 2,4, 2,5, 2,3, 1,2
1Department of Plant and Wildlife Sciences, Brigham Young University, 2Department of Entomology, Cornell University, 3Department of Molecular Biology and Genetics, Cornell University, 4Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, 5Biological Sciences, SUNY Oswego

Summary
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July 30th, 2016

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En metode til opdræt Drosophila melanogaster under Axeniske og gnotobiotiske betingelser præsenteres. Fly embryoner dechorionated i natriumhypochlorit, overføres aseptisk til sterilt kost, og opdrættet i lukkede beholdere. Pode kost og embryoner med bakterier fører til gnotobiotiske foreninger, og bakteriel tilstedeværelse bekræftes ved plating hele kroppen Drosophila homogenater.

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