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Journal / Developmental Biology / Het grootbrengen de fruitvlieg
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JoVE Journal Developmental Biology
Het grootbrengen de fruitvlieg
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Het grootbrengen de fruitvlieg

Article DOI: 10.3791/54219-v • 09:34 min • July 30th, 2016
July 30th, 2016 •
1, 1, 1, 2,4, 2,5, 2,3, 1,2
1Department of Plant and Wildlife Sciences, Brigham Young University, 2Department of Entomology, Cornell University, 3Department of Molecular Biology and Genetics, Cornell University, 4Division of Infectious Diseases, Boston Children's Hospital, Harvard Medical School, 5Biological Sciences, SUNY Oswego

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Een methode voor het kweken van Drosophila melanogaster onder axenic en gnotobiotische voorwaarden wordt gepresenteerd. Fly embryo's worden dechorionated in natriumhypochloriet, aseptisch in steriele voeding en grootgebracht in gesloten containers. Inenten van voeding en embryo's met bacteriën leidt tot gnotobiotische verenigingen, en bacteriële aanwezigheid wordt bevestigd door plating whole-body Drosophila homogenaten.

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Developmental Biology axenic gnotobiotische, Microbiome microbiota,
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