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Isolamento de perivascular Multipotentes populações de células precursoras a partir de tecido cardíaco humano
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Isolamento de perivascular Multipotentes populações de células precursoras a partir de tecido cardíaco humano

Article DOI: 10.3791/54252-v • 08:15 min • October 8th, 2016
October 8th, 2016 •
1, 2,3, 4, 4, 4, 5,6, 4,7
1Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery, University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering, Massachusetts Institute of Technology, 4MRC Centre for Regenerative Medicine, University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, 6Department of Orthopaedic Surgery, University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine, University of California at Los Angeles

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tecido cardíaco humano abriga populações de células precursoras perivasculares multipotentes que podem ser adequados para a regeneração do miocárdio. A técnica aqui descrita permite o isolamento e purificação simultânea de duas populações de células estromais multipotentes associados com os vasos nativos de sangue, ie CD146 + CD34 - pericitos e células CD34 + CD146 - células adventícias, a partir do miocárdio humano.

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Biologia do Desenvolvimento edição 116 de pericitos células adventícia vasos sanguíneos células-tronco células progenitoras células precursoras cardíaco o miocárdio regeneração cardíaca citometria de fluxo
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