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JoVE Journal Developmental Biology
Isolering af Perivaskulær multipotente precursorcelle populationer fra humant hjertevæv
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Isolering af Perivaskulær multipotente precursorcelle populationer fra humant hjertevæv

Article DOI: 10.3791/54252-v • 08:15 min • October 8th, 2016
October 8th, 2016 •
1, 2,3, 4, 4, 4, 5,6, 4,7
1Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, 2Department of Bioengineering and Orthopaedic Surgery, University of Pittsburgh, 3Research Laboratory of Electronics and Department of Biological Engineering, Massachusetts Institute of Technology, 4MRC Centre for Regenerative Medicine, University of Edinburgh, 5Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, 6Department of Orthopaedic Surgery, University of Texas Health Science Center at Houston, 7Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine, University of California at Los Angeles

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Humant hjertevæv huser multipotente perivaskulære precursor cellepopulationer, som kan være egnede til myocardial regenerering. Den her beskrevne teknik muliggør den samtidige isolering og oprensning af to multipotente stromale cellepopulationer forbundet med native blodkar, dvs. CD146 + CD34 - pericytter og CD34 + CD146 - adventitiaceller celler, fra den humane myocardium.

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Developmental Biology pericyt adventitial celle blodkar stamcellefaktor progenitorcelle hjerte- precursorcelle myocardium cardiac regeneration flowcytometri
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