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Un protocollo dettagliato per caratterizzare la linea cellulare murina C1498 e il suo modello associato leucemia mouse
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Un protocollo dettagliato per caratterizzare la linea cellulare murina C1498 e il suo modello associato leucemia mouse

Article DOI: 10.3791/54270-v • 08:00 min • October 14th, 2016
October 14th, 2016 •
1, 1, 1,2
1Institut pour la Recherche sur le Cancer de Lille (IRCL), INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC), 2Université de Lille

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Questo manoscritto fornisce una procedura tecnica che può essere utilizzato per caratterizzare colture cellulari C1498 in vitro e la leucemia acuta indotta nei topi dopo l'iniezione. Fenotipiche e funzionali analisi vengono eseguite utilizzando citometria a flusso, microscopia a immunofluorescenza, citochimica e maggio-Grünwald Giemsa.

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Cancer Research numero 116 Leucemia mieloide acuta linea cellulare topi midollo osseo milza timo citometria a flusso maggio-Grünwald Giemsa immunofluorescenza microscopia esterasi citochimica
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