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Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Article DOI: 10.3791/54307-v • 11:16 min • August 7th, 2016
August 7th, 2016 •
1,2,3,4, 3,4,5
1South Western Sydney Clinical School, Faculty of Medicine, University of New South Wales Australia, 2School of Medicine, Western Sydney University, 3Correlative Microscopy Group, Ingham Institute for Applied Medical Research, 4Electron Microscopy Laboratory, Department of Anatomical Pathology, Sydney South West Pathology Service, New South Wales Health Pathology, 5School of Medical Sciences, Faculty of Medicine, University of New South Wales Australia

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A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.

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Correlative Light Electron Microscopy Quantum Dot Nanoparticles Fluorescence Immunocytochemistry Ultra-structural Morphology Transmission Electron Microscopy CLEM Data Subcellular Structure Protein Of Interest Quantum Dot Nanoparticle Probe Live Microscopy Signal Electron Microscopy Structural Localization Fixing Embedding Sectioning Somatostatinoma Tumor Tissue Section Adhesive Solution Acetone Nickel Grid Etching Time Resin Formulation Sodium Metaperiodate Etching Solution
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