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JoVE Journal Bioengineering
Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold
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Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Article DOI: 10.3791/54331-v • 10:18 min • August 27th, 2016
August 27th, 2016 •
1, 1, 1, 1, 1, 1, 1, 1,2
1School of Materials Science and Engineering, Nanyang Technological University, 2School of Chemical and Biomedical Engineering, Nanyang Technological University

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This manuscript presents a detailed protocol for the fabrication of an emerging three-dimensional hepatocyte culture platform, the inverted colloidal crystal scaffold, and the concomitant techniques to assess hepatocyte behavior. The size-controllable pores, interconnectivity and ability to conjugate extracellular matrix proteins to the poly(ethylene glycol) (PEG) scaffold enhance Huh-7.5 cell performance.

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Inverted Colloidal Crystal Scaffold PEG-based Scaffold Liver Tissue Engineering 3D Cell Culture Platform Extracellular Matrix Drug Testing Stem Cells Micro-bead Molds Polystyrene Microspheres Ethanol Solution
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