Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Hitomi Shirahama; Supriya K. Kumar; Won-Yong Jeon; Myung Hee Kim; Jae Ho Lee; Soon Seng Ng; Seyed R. Tabaei; Nam-Joon Cho

doi:10.3791/54331

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Fabbricazione di invertito colloidale cristallo poli (glicole etilenico) Scaffold: una piattaforma Cell Culture tridimensionale per il fegato Tissue Engineering

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Fabbricazione di invertito colloidale cristallo poli (glicole etilenico) Scaffold: una piattaforma Cell Culture tridimensionale per il fegato Tissue Engineering

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9,833 Views

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10:18 min

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August 27, 2016

DOI:

10.3791/54331-v

DOI:

10.3791/54331-v

•

10:18 min

August 27, 2016

•

1 Views

Hitomi Shirahama¹, Supriya K. Kumar¹, Won-Yong Jeon¹, Myung Hee Kim¹, Jae Ho Lee¹, Soon Seng Ng¹, Seyed R. Tabaei¹, Nam-Joon Cho^1,2

¹School of Materials Science and Engineering,Nanyang Technological University, ²School of Chemical and Biomedical Engineering,Nanyang Technological University

Transcript

Summary

This manuscript presents a detailed protocol for the fabrication of an emerging three-dimensional hepatocyte culture platform, the inverted colloidal crystal scaffold, and the concomitant techniques to assess hepatocyte behavior. The size-controllable pores, interconnectivity and ability to conjugate extracellular matrix proteins to the poly(ethylene glycol) (PEG) scaffold enhance Huh-7.5 cell performance.