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Journal / Neuroscience / SNARE-mediert Fusion Single Proteoliposomes med…
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Neuroscience

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JoVE Journal Neuroscience
SNARE-mediert Fusion Single Proteoliposomes med tethered Støttede bilagene i en mikrofluid Flow Cell overvåkes av polarisert TIRF Mikroskopi
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SNARE-mediert Fusion Single Proteoliposomes med tethered Støttede bilagene i en mikrofluid Flow Cell overvåkes av polarisert TIRF Mikroskopi

Article DOI: 10.3791/54349-v • 10:58 min • August 24th, 2016
August 24th, 2016 •
1,2, 1,2,3,4
1Department of Cellular and Molecular Physiology, Yale University School of Medicine, 2Nanobiology Institute, Yale University, 3Department of Molecular Biophysics and Biochemistry, Yale University, 4Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales, Centre National de la Recherche Scientifique (CNRS)

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Her presenterer vi en protokoll for å detektere enkelt, skarptromme-mediert fusjonshendelser mellom liposomer og støttet bilagene i microfluidic kanaler ved hjelp av polarisert TIRFM, med enkelt molekyl følsomhet og ~ 15 ms tidsoppløsning. Lipid og løselig last utgivelsen kan oppdages samtidig. Liposomstørrelseforandringer, lipid diffusivitet, og fusion pore egenskaper måles.

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Neuroscience Membran fusion skarptromme proteiner lipid bilaget proteoliposomes støttet bilaget single vesikkelfusjon fusion pore eksocytose single-molekyl fluorescens MicroFluidics TIRFM biofysikk
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