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JoVE Journal Immunology and Infection
Voorbereiding en Toepassingen van organotypische thymus Slice Culturen
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Voorbereiding en Toepassingen van organotypische thymus Slice Culturen

Article DOI: 10.3791/54355-v • 10:10 min • August 6th, 2016
August 6th, 2016 •
*1,2, *1,2, 1,3
1Centre de Recherche, Hôpital Maisonneuve-Rosemont, 2Department of Microbiology, Infectiology and Immunology, Université de Montréal, 3Department of Medicine, Université de Montréal
* These authors contributed equally

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We beschrijven de bereiding van thymus segmenten die in combinatie met flowcytometrie, kan worden gebruikt om positieve en negatieve selectie van T-cellen ontwikkelen modelleren. Thymus segmenten kunnen ook worden aangepast voor de in situ analyse van thymocyten migratie, lokalisatie en signalering via immunofluorescentie en twee-foton microscopie.

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Immunologie thymus plakken orgelcultuur thymus positieve selectie de negatieve selectie T-cel ontwikkeling.
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