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JoVE Journal Neuroscience
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Fluorescentie Activated Cell Sorting (FACS) en genexpressie analyse van Fos expressie neuronen van verse en diepgevroren Rat Brain Tissue

Article DOI: 10.3791/54358-v • 08:37 min • August 27th, 2016
August 27th, 2016 •
*1, *1, 1, 2, 1
1Behavioral Neuroscience Research Branch, Intramural Research Program, NIDA, NIH, DHHS, 2Division of Rheumatology, School of Medicine, Johns Hopkins University
* These authors contributed equally

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Hier presenteren we een Fluorescence Activated Cell Sorting (FACS) protocol om moleculaire veranderingen studeren in Fos-uiting van neuronale ensembles van zowel verse als bevroren hersenweefsel. Het gebruik van bevroren weefsel maakt FACS isoleren van vele hersengebieden over meerdere zittingen aan het gebruik van kostbare proefdieren maximaliseren.

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Neuroscience Cell types neuronen glia antilichaam mRNA FACS
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